Journal of Next Generation Sequencing & Applications

ISSN - 2469-9853

+44 1250400002


DNA is Isolated and Partially Digested with Restriction Enzymes

Shuang-yong Xu*

Paired-cease a technique based totally on series-tagged connectors advanced to facilitate de novo genome sequencing to become aware of immoderate-decision reproduction amount and structural aberrations along with inversions and translocations. Short, the target genomic DNA is and partly digested with restriction enzymes into massive fragments. Following periodfractionation, the fragments are cloned into plasmids to assemble artificial chromosomes including bacterial artificial chromosomes which can be then sequenced and in comparisonto the reference genome.