De Mandal S, Panda AK, Bisht SS, Kumar NS
Microorganisms are the major part of earth’s biological diversity. Due to most of the microbes being nonculturable, it is necessary to use culture independent techniques to study the uncultured microbes. Metagenomics is the molecular tool which helps to understand the genetic makeup of the wide variety of uncultivable microorganisms. Currently, Next Generation Sequencing (NGS) is one of the most advanced technologies used in metagenomics studies and different computational tools have been developed for the analysis of large metagenomic dataset. This review demonstrates the different tools used in NGS analysis and its applications in microbial ecology.
