When found, collected, and correctly recognised, body fluids can be a valuable tool in forensic investigation. Numerous techniques, including those based on serology, were employed over a long period of time, but it was difficult to ignore their lack of sensitivity and specificity. MiRNA profiling exploded with a great potential to be utilised to identify evidences such urine, blood, menstrual blood, saliva, semen, and vaginal secretions in order to divert attention from the issue. MiRNAs are 20 nt–25 nt short RNA structures that have characteristics that make them less susceptible to breakdown processes than mRNA. This is crucial since biological evidence may be exposed to various harmful environmental elements at a crime scene. A few particular miRNAs have recently been identified by published research, although their findings were not always repeatable by others, which may be the result of varied workflow techniques used for their profiling studies. Given the present surge in interest in miRNAs, it is crucial to recognise any potential limitations of miRNA profiling, however there aren't any research that clearly demonstrate this. . This review makes the claim that it has compiled all available data and evaluated a wide range of variables that could potentially cast doubt on miRNA profiling, including methodological approaches, environmental factors, physiological conditions, gender, diseases, and sample preservation. Although significant progress could yet be achieved, we pretend to emphasise one conceivable response to the fundamental query: Is miRNA profiling a forensic biomarker for identifying bodily fluids?
