Journal of Next Generation Sequencing & Applications

ISSN - 2469-9853

+44 1250400002


Sanger Sequencing is a Strategy for DNA

Marie Umber

Sanger sequencing is a strategy for DNA sequencing dependent on the specific consolidation of chain-ending dideoxy nucleotides by DNA polymerase during in vitro DNA replication. After first being created by Frederick Sanger and partners in 1977, it turned into the most broadly utilized sequencing technique for roughly 40 years. It was first marketed by Applied Bio systems in 1986. More as of late, higher volume Sanger sequencing has been supplanted by "Cutting edge" sequencing strategies, particularly for huge scope, robotized genome examinations. In any case, the Sanger strategy stays in wide use, for more limited size projects, and for approval of Next-Gen results. It actually has the benefit over short-read sequencing advancements (like Illumina) in that it can create DNA succession peruses of > 500 nucleotides.