Journal of Next Generation Sequencing & Applications

ISSN - 2469-9853

+44 1250400002


Shotgun Sequencing in Human Genetics

Nandita R. Garud*

In genetics, shotgun sequencing is a method used for sequencing random DNA strands. It is named by using analogy with the unexpectedly expanding, quasi-random shot grouping of a shotgun. The chain-termination approach of DNA sequencing Sanger sequencing can simplest be used for quick DNA strands of 100 to 1000 base pairs. Due to this size restrict, longer sequences are subdivided into smaller fragments that may be sequenced one after the other, and these sequences are assembled to offer the overall collection. There are main techniques for this fragmentation and sequencing technique