ijddr

International Journal of Drug Development and Research

ISSN - 0975-9344

- (2011) Volume 3, Issue 1

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Antimicrobial Investigation on Manilkara zapota (L.) P. Royen

 

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Abstract

The present study was designed to screen antimicrobial activity of Manilkara zapota (L.). Bioassays for antimicrobial activities were carried out using ethyl acetate extracts of both stem bark and leaves of Manilkara zapota against some pathogenic bacteria and fungi. TLC (Thin layer chromatography) profile of isolated extracts showed the presence of terpenoids, glycosides and flavoniods type compounds. The ethyl acetate extract of stem bark exhibited antimicrobial activity against all the pathogenic bacteria used in this study and also showed activity against Aspergillus flavus, Vasianfactum sp and Fusarium sp. with inhibition zones in the range of 08-16 mm. Ethyl acetate extract of leaves possessed mild activity against Bacillus subtilis, Bacillus megaterium, Sarcina lutea, Escherichia coli and Salmonella typhi. The minimum inhibitory concentrations (MICs) of the extracts were found to be in the range of 256 ~ 512 ìg/ml. The cytotoxicity (LC50) against brine shrimp nauplii (Artemia salina) were also evaluated and found to be 16.17 ìg/ml for ethyl acetate extract of leaves, 50.26 ìg/ml for ethyl acetate extract of stem bark and 12.38 ìg/ml for ampicillin trihydrate.

References

  1. Benkeblia N. Antimicrobial activity of essential oil extracts of various onions (Allium cepa) and garlic (Allium sativum). Turk J Biol 2004; 37: 263-268.
  2. Zakaria M. Isolation and characterization of active compounds from medicinal plants. Asia Pac J Pharmacol 1991; 6: 158-20.
  3. Parekh J, Chanda S. In vitro antibacterial activity of the crude methanol extract of Woodfordiafruticosakurz. flower (Lythraceae). Braz J Microbiol 2007; 38: 204-207.
  4. Shui G, Wong SP, Leong LP. Characterization of antioxidants and change of antioxidant levels during storage of Manilkarazapota L. J Agric Food Chem 2004; 52(26): 7834-7841.
  5. Ghani A. Medicinal Plants of Bangladesh with chemical constituents and uses (2nd edn), Asiatic Society of Bangladesh, Dhaka, 2003, pp. 292.
  6. Ma J, Luo XD, Protiva P, Yang H, Ma C, Basile MJ, Weinstein IB, Kennelly EJ. Bioactive novel polyphenols from the fruit of Manilkarazapota L. (Sapodilla). J Nat Prod 2003; 66(7): 983-986.
  7. Harborne JB. Phytochemical methods (3rd edn) Chapman and Hall, London, 1978, pp. 135, 203.
  8. Rois JJ, Reico MC, Villar A. Antimicrobial Screening of natural products, J Enthopharmocol 1988; 23: 127-149.
  9. Rois JJ, Reico MC, Villar A. Antimicrobial Screening of natural products. J Enthopharmocol 1988; 23: 127-149.
  10. Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, Mclaughlin JL. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med 1982; 45: 31-33.
  11. Finney DJ. Probit Analysis. Cambridge University Press, London, 1971, pp: 333.
  12. Kumaraswamy MV, Kavitha HU, Satish S. Antibacterial potential of extracts of Woodferdiafruticosakurz on human pathogens. World J Med Sci 2008; 3(2): 93-96.
  13. Viji M, Murugesans S. Phytochemical analysis and antibacterial activity of medicinal plant Cardiospermumhalicacabum Linn. Journal of Phytol 2010; 2(1): 68-77.
  14. Mandalari G, Bennett RN, Bisignano G, Trombetta D, Saija A, Faulds CB, Gasson MJ, Narbad A. Antimicrobial activity of flavonoids extracted from bergamot (Citrus bergamiaRisso) peel, a byproduct of the essential oil industry. J ApplMicrobiol 2007; 103(6): 2056-2064.
  15. Maiyo ZC, Ngure RM, Matasyoh JC, Chepkorir R. Phytochemical constituents and antimicrobial activity of leaf extracts of three Amaranthus plant species. Afr J Biotech 2010; 9(2): 3178-3182.
  16. Quignard EL, Pohlit AM, Nunomura SM, Pinto AC, Santos EV, Morais SK, Alecrim AM, Pedroso AC, Cyrino BR, Melo CS, Finney EK, Gomes EO, Souza KS, Oliveira LC, Don LC, Silva LF, Queiroz MM, Henrique MC, Santos M, Pinto PS, Silva SG. Screening of plants found in Amazonas state for lethality towards brine shrimp.Acta Amazon 2003; 33: 93-104

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