ijddr

International Journal of Drug Development and Research

ISSN - 0975-9344

- (2011) Volume 3, Issue 3

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INVITRO ANTI-INFLAMMATORY ACTIVITY OF METHANOL EXTRACT OF ENICOSTEMMA AXILLARE

 

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Abstract

Methanol extract of whole plant of Enicostemma axillare (Family: Gentianaceae) was assessed for its anti-inflammatory activity by invitro methods. Invitro anti-inflammatory activity was evaluated using albumin denaturation assay, proteinase inhibitory activity, membrane stabilization, and anti-lipoxygenase activity at different concentrations. Aspirin, Diclofenac sodium, Indomethacin were used as standard drugs. The results showed that Enicostemma axillare Methanol Extract (EAME) at a concentration range of 100-500μg/ml significantly (p<0.01) protects the heat induced protein denaturation. At the concentration of 400 and 500 μg/ml, EAME showed significant (p<0.01) inhibition of 42 and 53% of proteinase inhibitory action, but at the concentration of 100 and 200 μg/ml did not show significant (p>0.05) activity. Heat induced haemolysis of erythrocyte was significantly (p<0.05) inhibited at the concentration of 400 and 500μg/ml. Hypotonicity induced haemolysis and lipoxygenase activity were significantly (p<0.01) inhibited at the concentration range of 200-500μg/ml and 400, 500μg/ml respectively. The results obtained in the present study indicate that methanol extracts of Enicostemma axillare can be a potential source of anti-inflammatory agents.

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Copyright:This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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