Chi Zhang
Tanzania
Review Article
Bioinformatics Tools for RNA-seq Gene and Isoform Quantification
Author(s): Chi Zhang, Baohong Zhang, Michael S Vincent and Shanrong ZhaoChi Zhang, Baohong Zhang, Michael S Vincent and Shanrong Zhao
In recent years, RNA-seq has emerged as a powerful technology in estimation of gene or transcript expression. ‘Union-exon’ and transcript based approaches are widely used in gene quantification. The ‘Union-exon’ based approach is simple, but it does not distinguish between isoforms when multiple alternatively spliced transcripts are expressed from the same gene. Because a gene is expressed in one or more transcript isoforms, the transcript based approach is more biologically meaningful than the ‘union exon’-based approach. However, estimating the expression of individual isoform is intrinsically challenging because different isoforms of a gene usually have a high proportion of genomic overl.. View More»
