Robinson C Jose
Institute of Advanced Study in Science and Technology,
Guwahati 781035, Assam
India
Short Communication
Confined Enzymatic Activity of S. scitamineum and U. esculenta at the Smut Gall during Infection
Author(s): Robinson C Jose, Nongthongbam D Grihalakshmi, Bengyella Louis and Narayan C TalukdarRobinson C Jose, Nongthongbam D Grihalakshmi, Bengyella Louis and Narayan C Talukdar
We aimed to evaluate the enzymatic abilities of U. esculenta and S. scitamineum to degrade cellulose, pectin and lignin and explain the possible confinement of the smut - fungi in the smut-gall. S. scitamineum and U. esculenta were isolated from the smut gall of S. spontaneum and Z. latifolia plants, respectively. The teliospores were inoculated into a culture flask containing various concentrations (3%-10%) of pure cellulose powder to check the degradation ability of the fungi. The fungi were sub-cultured onto modified Czapek Dox Agar (CDA) supplemented with 0.3% carboxy methyl cellulose (CMC) followed by Gram’s Iodine test to determine the relative cellulolytic activity index (RCAI). Extracellular proteins were harvested and profiled by SDS-PAGE and subsequently zymography was carried out with regular 0.1% substrate infiltr.. View More»
