Simon Gross
Australia
Research Article
DPP4 Inhibition in Human Kidney Proximal Tubular Cells - Renoprotection in Diabetic Nephropathy?
Author(s): Usha Panchapakesan, Simon Gross, Murali Gangadharan Komala, Kate Pegg and Carol A PollockUsha Panchapakesan, Simon Gross, Murali Gangadharan Komala, Kate Pegg and Carol A Pollock
Novel diabetic drugs that target the incretin system e.g dipeptidyl peptidase 4 (DPP4) inhibitors have pleiotropic effects other than inhibiting the cleavage of glucagon like peptide 1 (GLP-1). As DPP4 is located at the brush border of the kidney proximal tubular cells (PTC), we hypothesise that it is involved in the processing of luminal peptides influencing the kidney tubulointerstitium in diabetes. Using HK2 cells (human kidney proximal tubular cell line) in order to study the GLP-1 independent effects of DPP4 inhibition on the PTC cells were exposed to high glucose conditions, high mobility protein 1(HMGB1) and meprin beta. Relevant markers like DPP4, fibronectin, collagen IV, transforming growth factor beta (TGFβ), activator protein 1(AP-1) and nuclear factor-kappa B (NF-κB ) were measured. We show tha.. View More»
