Yizhe Cui
China
Research Article
Role of Nuclear Factor Erythroid 2-Like 2 in the Induction of Cytochrome P450 2a5 in Vivo of Nonalcoholic Fatty Liver Disease
Author(s): Yizhe Cui, Qiuju Wang, Chunhua Wang, Xing Yi, Yue Qi, Guangliang Li and Xiuying ZhangYizhe Cui, Qiuju Wang, Chunhua Wang, Xing Yi, Yue Qi, Guangliang Li and Xiuying Zhang
Mouse cytochrome P450 2A5 (Cyp2a5) is upregulated in various pathophysiological liver disease and induced by structurally variable hepatotoxic chemicals. A putative common feature for all of these conditions is altered cellular redox status. Nuclear factor erythroid 2-like 2 (Nrf2) is a transcription factor that is post-translationally regulated by oxidative stress and controls the transcription of numerous protective target genes. In order to determine if upregulation of Cyp2a5 by Nonalcoholic fatty liver disease (NAFLD) was mediated through Nrf2, we applied Nrf2 knockout mice (Nrf2-/-) to examine the Cyp2a5 mRNA and protein levels compared to wild type mice. Furthermore, the interaction of Nrf2 with Cyp2a5 was confirmed by co-immunoprecipitation (co-IP) in vivo. The NAFLD increased the Nrf2 mRNA and protein levels i.. View More»
