Department of Biochemistry, J.N. Medical College, Faculty of Medicine, Aligarh Muslim University, Aligarh, India
Opinion
The autoimmune response to AGE-modified human DNA and its implications for type 1 diabetic mellitus
Author(s): Musraf Uddin*
Aims: Both in vivo and in vitro non-enzymatic glycation of DNA produces
free radicals, or glycoxidation. Neo-antigenic epitopes, which play a role
in autoimmune diseases like diabetes mellitus, are produced as a result
of DNA structural alterations caused by glycolysis. In this study, autoantibody
binding to human placental DNA was probed in Type 1 diabetes
patients after it was glycated with methylglyoxal (MG) and lysine (Lys) in
the presence of Cu2+.
Methods: DNA was lysed by incubating it for 24 hours at 37 °C with MG,
Lys, and Cu2+. LC-MS and ESI-MS techniques were used to investigate
the pathway for Amadori formation and the carboxyethyl deoxyguanosine
(CEdG) formation during the glycation reaction. In addition, a direct binding,
competitive ELISA, and band shift assay were used to evaluate the autoantibo.. View More»
DOI:
10.35248/2155-6156.1000989
