Fatima Mahmoudi and Miloud Hedadji
Posters-Accepted Abstracts: Biol Syst Open Access
Bifidobacteria play an important role in maintaining human health and suppress harmful bacteria by controlling the pH of the large intestine. About 14 strains of the Bifidobacteria have been isolated from the feces of infants nursed exclusively from the maternal milk (their ages less than 5 months) from commercial French yoghurt (active bifidus) and from milk (celiabifidus) on MRS medium (containing 0.05% cysteine-HCl with nalidixic acid 2 mg m-1). A total of six strain are strict anaerobic and gram-positive. Bifidobacteria identified as B. longum (60% of the samples) was the most frequently found species followed by B. bifidum (21.0%), B. breve (19%). These strains were evaluated for potential use as probiotics based on their adhesion to intestinal epithelial cells, resistance towards acidic (resistance to low pH) and bile conditions. pH values ranging from 6.5 to 7, however, these strains are sensitive to the pH 8, Bifidobacterium are mesophiles with optimal growth at 36°C to 41°C. All isolates studied have a fast growth in glucose.
