Komal H. Advani, Rashmi S. Tupe, Vaishali D. Mittal, Pavani S. Narayanam, Kamlesh B. Mahajan and Arundhati G. Diwan
Posters: J Diabetes Metab
The complex interplay between glycation and oxidative stress forms an integrated vicious cycle because reactive oxygen species in diabetes accelerates glycation reaction and collectively referred to as ?glycoxidation?. This subsequently contributes to the progression of vascular complications in diabetes. The aim of present study was to investigate the levels of glycoxidation markers in 70 type 2 diabetic and 40 healthy control. Various markers were assessed for glycoxidation such as protein carbonyls, thiols, nitric oxide and total antioxidant capacity. Protein carbonyls levels were significantly amplified (p<0.001) in diabetic patients as carbonyl level was 21.23 nmol/mg protein as compare to 18.84 nmol/mg protein in controls. Protein thiols the major antioxidant in plasma was found to be decreased in diabetic patients 1.16 nmol/mg protein as compare to 1.36 nmol/mg protein in controls (p<0.001). The plasma total antioxidant capacity as assayed by the FRAP method was found to be 24.63 ? 9.2 μmol in diabetic patients, which is significantly lower (p<0.001) than that of control subjects (32.87 ? 14.6 μmol). Additionally decreased nitric oxide levels were found in diabetic patients than controls. Cumulatively, these results demonstrate the complex interplay between glycation, glycoxidation and oxidative stress generation in type 2 diabetes as the glycated proteins eventually degrade due to glycoxidation. Thus, the overall results emphasize the induced glycatory and glycoxidation modifications of plasma proteins due to type 2 diabetic conditions.
Komal H.Advani is a final year M.Sc.Biotechnology student doing her thesis/ dissertation project under Dr. Rashmi Tupe at Rajiv Gandhi Institute of Information technology and Biotechnology, Bharti Vidyapeeth Deemed University Pune.
