S R Kulkarni, Vanita Kale and Shruti Raut
Bombay College of Pharmacy, India
Posters-Accepted Abstracts: Nat Prod Chem Res
Pheophorbide are the product of chlorophyll breakdown. We extracted chlorophyll from spinach and derivatized it to pheophorbide by chlorophyllase enzyme and further addition of 1N HCl. They can be prepared by synthetic and semi-synthetic ways. We prepared ethosomes of pheophorbides. They are formed by following method. Blank Ethosomal: 100 mg of Phospholipon 90 G was dissolved in Ethanol (1000 mg). Filtered distilled water was added slowly as fine stream using a syringe to ethanolic solution of Phospholipon 90 G with constant stirring using magnetic stirrer at 700 rpm. Mixing was continued for 4 hours. Ethosomes prepared by above procedure were subjected to 3 sonication cycle of 5 minutes each with 5 minutes break between two cycles. For Drug loaded Ethosomal, 1% and 10% w/w Pheophorbides were dissolved in ethanol with Phospholipon 90 G and above procedure was followed. Ethosomal vesicles prepared by above mentioned methods were observed visually under microscope. The Ethosomal vesicles have shown pheophorbide entrapped in them. The amount of loading of pheophorbides can be studied by HPTLC and any other suitable technique.
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