Mohamed Abu-Farha
Scientific Tracks Abstracts: J Diabetes Metab
The modern increase in sedentary lifestyles and excessive food intake are considered as key contributing factors to a group of obesity-associated disorders such as insulin resistance, diabetes, and cardiovascular complications. Proteomic profiling strategy was used to identify proteins that are differentially expressed and might play a role in obesity and the potential modulation of their expression by physical exercise. PBMCs and subcutaneous adipose tissue biopsies from 48 male non-diabetic subjects were collected before and after exercise. Proteins were extracted from PBMCs, and digested SCX beads and analyzed by LC-Orbitrap Velos. Label-free method was then used to quantify identified proteins. Data were validated by Q-PCR and immunohistochemistry. Circulating cytokine and metabolic markers were investigated by multiplexing technology.
