Biological Systems: Open Access

ISSN - 2329-6577


The effect of probiotic stains (Bifidobacterium bifidum) on intestinal microbiota and contamination with enteropathogenic E. coli (EPEC)

3rd International Conference on Integrative Biology

August 04-06, 2015 Valencia, Spain

F Mahmoudi and M Hadadji

Posters-Accepted Abstracts: Biol Syst Open Access

Abstract :

This study illustrates the importance of antimicrobial Bifidobacterium sp. against the enteropathogens. Six strains of bifidobacteria belonging to the species B. bifidum, B. longum and B. breve were isolated on MRS medium supplemented with cysteine MRS 0.05% cysteine chlorhidrique and 2 mg/l nalidixic acid from stools of infants and yogurt (Activia) and saline rehydration (celia) the study of the physiological characteristics of the viruses (Bbf1, B2, B3, BV, B4, RBL8). The study of the kinetics of growth in mixed culture allowed us to choose a strain of B. bifidum strain as a model in vivo in the rat, watch it there is a strong possibility that the strain has resisted the gastrointestinal passage. Similarly, in this work we also records that the administration of Bifidobacterium strain cause a significant reduction in the rate of enteropathogenic E. coli in the feces of rats and the results of the macroscopic study of histological sections showed the rats in one lot who have not received B. bifidum displayed symptoms of severe intestinal infection accompanied by a marked contraction of the intestinal lumen (intestinal atrophy) probably due to E. coli contamination. These results have disappeared (batch 3) and after treatment with bifidobacteria or did not even appear (batch 2) in rats that received B. bifidum. The in vitro and in vivo study showed that taking probiotics (such as Bifidobacterium) reduces colonization of the digestive tract by pathogenic bacteria.