Narayan C Talukdar
India
Short Communication
Confined Enzymatic Activity of S. scitamineum and U. esculenta at the Smut Gall during Infection
Author(s): Robinson C Jose, Nongthongbam D Grihalakshmi, Bengyella Louis and Narayan C TalukdarRobinson C Jose, Nongthongbam D Grihalakshmi, Bengyella Louis and Narayan C Talukdar
We aimed to evaluate the enzymatic abilities of U. esculenta and S. scitamineum to degrade cellulose, pectin and lignin and explain the possible confinement of the smut - fungi in the smut-gall. S. scitamineum and U. esculenta were isolated from the smut gall of S. spontaneum and Z. latifolia plants, respectively. The teliospores were inoculated into a culture flask containing various concentrations (3%-10%) of pure cellulose powder to check the degradation ability of the fungi. The fungi were sub-cultured onto modified Czapek Dox Agar (CDA) supplemented with 0.3% carboxy methyl cellulose (CMC) followed by Gram’s Iodine test to determine the relative cellulolytic activity index (RCAI). Extracellular proteins were harvested and profiled by SDS-PAGE and subsequently zymography was carried out with regular 0.1% substrate infiltr.. View More»
