Shigeto Hamaguchi
Japan
Research Article
PCR-based Method for Rapid and Minimized Electrochemical Detection of mecA Gene of Methicillin-resistant Staphylococcus aureus and Methicillinresistant Staphylococcus epidermidis
Author(s): Tomohiko Ikeuchi, Masafumi Seki, Yukihiro Akeda, Norihisa Yamamoto, Shigeto Hamaguchi, Tomoya Hirose, Keiichiro Yamanaka, Masato Saito, Kazunori Tomono and Eiichi TamiyaTomohiko Ikeuchi, Masafumi Seki, Yukihiro Akeda, Norihisa Yamamoto, Shigeto Hamaguchi, Tomoya Hirose, Keiichiro Yamanaka, Masato Saito, Kazunori Tomono and Eiichi Tamiya
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important pathogens that cause nosocomial infections. However, microbiological culture techniques take a few days to yield results; therefore, a simple, cost-effective, and rapid detection system is required for screening for MRSA and related bacteria: Methicillinresistant Staphylococcus epidermidis (MRSE) carriers during the hospital admissions process. In this study, we described the simplified method using by one-time use and screen-printed carbon electrodes, relied upon current quantification of Hoechst dyes which bound with DNA amplified via polymerase chain reaction (PCR) targeted for MRSA mecA gene. Amount of DNA-bound Hoechst molecules were measured by the hand-held potentiostat within two minutes. We found that the peak of a Hoechst-mediated current depended upon the number of MRSA cells, and successfully d.. View More»
