Natural Products Chemistry & Research

ISSN - 2329-6836


In Vitro Antimicrobial Efficacy of Fractions from Demakese (Ocimum lamifolium) Leaves Extract from Mekelle Tigray, Ethiopia

Teklit Gebregiorgis Amabye and Said Mussa

The aim of the study was to screen the antibacterial activities and phenolic compounds of methanol extracts of the Ocimum lamiifolium available in mekelle Tigray Ethiopia. Crude extract of the leaves methanol were screened for antibacterial activities against S. aureus, Pseudomonas spp. and Escherichia coli. The in vitro antibacterial activity was performed by agar disc diffusion method and this shows that methanol extract of Ocimum lamiifolium revealed an elevated antimicrobial activity against S. aureus, E. coli and Pseudomonas spp. The quantitative analysis of phenolic constituents was determined using high-performance liquid chromatography and shows that the phytochemicals found in each extract are rich antioxidants and these extracts can be used as an effective preservative in food industry. The results obtained in the present study suggest that the methanol extracts of Ocimum lamiifolium revealed a significant scope to develop a novel antibacterial herbal formulation and a source of antibacterial drugs.