Uday Venkatesh, Shiva Prasad Kollur, Chethan Javarashetty, Shankar Jayarama and Satish Kumar Murari
Herein, we report extraction of biologically important isolates from medicinally valuable plant Wedelia trilobata. The biological activities viz., anti-proliferative activity and cytotoxic effect of the methanolic extract of Wedelia trilobata was investigated by Thymidine uptake assay, wound healing assay and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, respectively. In addition, apoptotic activity was also examined through nuclear staining and DNA fragmentation assays. The IC50 value of the methanolic extract for the growth inhibition of MEG-01 cells was found to be 80 μg/ml under which the normal HEK-293 cells were resistant to the toxic effect. Concentration and time dependent studies in Thymidine uptake and wound healing assays revealed the prominent antiproliferative activity against MEG-01 at the concentration of 80 μg/ml for 48 h. The nuclear staining and DNA fragmentation assays also confirmed that the methanolic extract induces apoptosis in MEG-01 cells.