Natural Products Chemistry & Research

ISSN - 2329-6836


Phytochemical Investigations of Methanol Leaf Extracts of Randia spinosa Using Column Chromatography, HPTLC and GC-MS

Mahabaleshwara K, Chandrasekhar N and Govindappa M

The present study was aimed to identify phytochemical presence in leaves of Randia spinosa using hexane, chloroform, ethanol, methanol and distilled water for extraction. Among these solvents, methanol have shown important phytochemicals in qualitative method. Further, column chromatography has been carried out for the partial purification of methanol extract by sequential elution with hexane, ethyl acetate and methanol as eluents. The methanol extract of HPTLC study shows the significant phytochemicals. GC-MS result of ethyl acetate extraction of leaves shown the presence of triterpenoid esters, cinnamate, naphthalene, phenolic compounds mainly BHT and methanol fraction has shown the presence of triterpenoid esters, amines and coumarins mainly, 5a-methoxy-9a-methyl-3-(1-propenyl) perhydro-pyrano[4,3-b]benzopyran