Gusain P
India
Research Article
Micropropagation of Caralluma adscendens var. fimbriata-An Indigenous Medicinal Plant of India
Author(s): Ramachandra Naik M, Rajappa Joga J, Nagaraja N, Nagashree B, Shankramma N, Chanda P, Gusain P and Krishnamurthy YLRamachandra Naik M, Rajappa Joga J, Nagaraja N, Nagashree B, Shankramma N, Chanda P, Gusain P and Krishnamurthy YL
Present study describes micro-propagation of traditionally important medicinal plant in northern interior part of Karnataka, Caralluma adscendens var. fimbriata, for its conservation. Results showed that the survival ability of regenerated plants was 84 per cent. Proliferation of C. adscendens var. fimbriata shoots was achieved on medium supplemented with various concentrations of different hormones. The effective combination of 6-Benzylaminopurine (BA) 2.0 mg/l along with 0.5 mg/l kinetin (Kn) and 0.2 mg/l Indole-3-acetic acid (IBA) produced highest number of shoot sprouting frequency (91%) with a mean of 2.60 ± 0.17 shoot number per explants and a mean shoot length of 3.88 ± 0.20 cm from the mature nodal explants. A good frequency of callus was induced in 1.5 to 2.0 mg/l of 1-Naphthaleneacetic acid (NAA) and in 2.0 mg/l of 2,4-dichlorophenoxy acetic acid (2, 4-D) were .. View More»