Hanan M Elwy
Central America
Research Article
Leptadenia pyrotechnica Induces P53-Dependent Apoptosis in Colon Cancer Cells
Author(s): Mohammad A Khasawneh, Adrian Koch, Hanan M Elwy, Alaaeldin A Hamza and Regine Schneider- StockMohammad A Khasawneh, Adrian Koch, Hanan M Elwy, Alaaeldin A Hamza and Regine Schneider- Stock
Leptadenia pyrotechnica has been commonly used traditionally for the treatment of tumors in Gulf countries. However, the molecular mechanism of the anti-cancer activity of Leptadenia pyrotechnica remains unclear. In the present study, 80% ethanolic extract of Leptadenia pyrotechnica and its fractions (n-hexane, EA, n-butanol and aqueous) were evaluated for in vitro cytotoxicity against colon cancer cell lines (HCT 116 wild type and HCT 116 p53−/−). The hexane fraction of Leptadenia pyrotechnica (LHE) is identified as the major anti-cancer fraction in Leptadenia pyrotechnica solvent extracts. Cells were incubated with different concentrations of LHE. Cell viability was quantified by crystal violet and MTT assays. Apoptotic cells were determined using Annexin V-propidium iodide (Annexin V/PI.. View More»
