Department of Microbiology, Federal University of Agriculture, Abeokuta, Nigeria
Research Article
Vector Control and Isolation of Larvicidal Compounds from Melia Azedarach (A. Juss) Leaf Using Anopheles Gambiae Larva
Author(s): Olugbenga A Ojo* and Abidemi E Ojo
The aim of this present investigation is to evaluate and isolate larvicidal principles in leaves of Melia azedarach using the fourth instar larvae of An. gambiae. Larvicidal activity was evaluated by exposing 4th instar larvae of Anopheles gambiae to serial concentrations (0.625-10 mg/mL) of extracts, fractions and compounds of Melia azedarach. Larval mortality was recorded after 24h of exposure and 50% lethal concentration (LC50) values determined. L Results were compared to those of larvae exposed to N, Ndiethyl-m-toluamid (DEET), the reference insecticides and untreated groups. Larvicidal activity of the crude methanol extract of both leaves and stem bark of M. azedarach were 5.80 and 7.59 mg/mL respectively. The hexane soluble fraction of M. azedarach leaf displayed highly lethality at 0.625 mg/mL with LC50 of 0.025 than the remaining fractions. Similarly, larvicidal activity of D.. View More»
DOI:
10.37532/npcr.6.1.1-5
