Department of Applied Biology, School of Applied Natural Sciences, Adama Science and Technology University, P.O. Box 1888, Adama, Ethiopia
Research Article
Quantification of Bioactive Constituent D-Pinitol in Ethiopian Soybean
Author(s): Shimekit Tadele* and Solomon Girmay
Vegetable soybean (Glycine max) is well established legume in the human diet in all over the world. D-Pinitol is a natural product belonging to groups of Cyclitols. D-pinitol plays tremendous medicinal roles. The aim of this work is to quantify the bioactive constituent D-Pinitol from seeds, seed pods and leaves of soybean. D-Pinitol extraction of soybean was processed with methanolic extraction followed by quantification using a UV spectrophotometer in comparisons with the Caro pinitol®. The maximum wavelength and absorbance of the standard dissolved in laboratory reagent water were 193 nm and 0.904 respectively. The best-fit line, R2, was found to be greater than 0.9983, indicating the D-pinitol’s concentration has significant effect on D-pinitol’s absorbance over a concentration range of 31.25-1000.0 μg/mL. The mean recovery of the method was 94.3 ± 8.06%... View More»
DOI:
10.35248/2329-891X.18.6.313
