Natural Products Chemistry & Research

ISSN - 2329-6836

A reliable characterisation of Eugenol an anti-diabetic constituents from plant Myristica Fragrans by analytical technique

2nd International Conference and Exhibition on Marine Drugs and Natural Products

June 15-17, 2017 London, UK

Mohamad Taleuzzaman, Sadaf Jamal Gilani and Mohd. Aamir Mirza

Glocal University, India
Fulvic Limited, New Zealand

Posters & Accepted Abstracts: Nat Prod Chem Res

Abstract :

Today, there is a so many demand of herbal medicine in the global market but due to scarcity of authentic data regarding the parameters and methods employed for assessing the quality of medicines Myristica fragrans Houtt. Eugenol is present in many aromatic plants. It is reported 320 ppm is present in the seed of Myristica fragrans. It contains an active principle of drug identified and quantifies by chromatography method, and its structure was established by spectroscopic methods. To assess the quality of raw materials, proximate parameters like ash values (total ash, acid insoluble ash and water soluble ash), loss on drying and foreign matter were determined using standard pharmacopoeial methods as per WHO guidelines. A stock solution of Eugenol (1000μg/ml) was prepared by dissolving 10 mg accurately weighed Eugenol in methanol and diluting it to 10 ml of methanol. From this stock solution, a calibration curve is developed with a dilution series in range of concentration 0.2μg/ml -10μg/ml. From UV-Spectroscopy λmax determined and it is found to be 278-280 nm by scanning 200-400 nm. For method development study prepared three quality control dilutions, samples of 2, 6 and 8μg/ml were prepared for studying precision, accuracy and ruggedness. Eugenol belongs to a class of phenyl propanoids (C10H12O2). The IUPAC name of the compound is 4-Allyl-2-methoxyphenol having molecular mass 164.2g/mol with pKa=10.19 at 25°C. Apart from anti-diabetic properties, reported other pharmacological properties like hypocholesteremic, anti-inflammatory, anti-diarrheal, chemo preventive action, etc. and hence there is a great need to determine the amount of eugenol present in the different extracts. In vivo diabetic mice model studies with eugenol showed reduction in blood glucose levels by 38% likely due to inhibition of α-glucosidase while insulin and glycated hemoglobin levels remain unchanged. Diabetes Mellitus (DM) is a metabolic disorder of multiple etiologies characterized by elevated levels of blood glucose resulting from defects in insulin production. The proposed characterisation is helpful for further study in instrument like HPLC and LC-MS/MS.

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