D G Perez Ishiwara, A Martinez Cuazit, M D C Gomez Garcia, V Sanchez Monroy, E San Martin Martinez, M Rojas Lopez, R J Delgado Macuil and J S Salas Benito
Instituto Politécnico Nacional, Mexico
Medical Military School, Mexico
Posters & Accepted Abstracts: Nat Prod Chem Res
Skin wound healing progress is directed by complex coordination and interaction of several cell type soluble mediators and extracellular matrix. New alternatives for wound management have been sought. Previous results from our laboratory demonstrated that Bacopa Procumbens Green Mill Extract (BPE) enhance the wound healing process. The main objective of this work was to evaluate the molecular effectors and signaling pathway induced by BPE treatment in excisional in vivo model skin wound healing. Through immunohistochemistry and qRTPCR we evaluated the expression of molecular effectors and signaling pathways induced by the healing effect of BPE at 3, 5 and 7 days post-injury in vivo model. After 3 day BPE stimulates the expression of TGFβ 1 (2.18±0.06), smad 2/3 (1.39±0.085) ERK 1/2 (1.72±0.047) phosphorylated, MMP9 (13.1±1.16) and β-SMA (2. 52±0.104), all of them involved in the healing process. Interestingly since 5 day, BPE improved collagen type I expression restoring the levels founded in normal skin. Our results showed that BPE regulates different wound healing molecular effectors through TGF-β expression that regulates effects on cell growth, differentiation, migration, and extracellular matrix deposition; BPE effects suggests that it can regulates inflammatory phase by MMP9 overexpression, it also improved fibroblast differentiation and interestingly, BPE enhance extracellular matrix deposition.
Email: ishiwaramx@yahoo.com.mx
