Yi-Chen Liu, Chi-Chen Lin, Wen-Te Chang, Meng-Shiou Lee and Ming-Kuem Lin
National Chung-Hsing University, Taiwan
China Medical University, Taiwan
Posters & Accepted Abstracts: Nat Prod Chem Res
Scrophularia yoshimura (Scrophulariaceae) is a Taiwan endemic plant. It has been proved that its main active compounds are higher than Scrophularia ningpoensis (Chinese figwort, xuán cān). Transformed hairy root which grows rapidly is used to produce large quantity of active compounds for many medicinal plants. However, there is no research about different wavelengths of light influence transformed hairy root. The aim of this study is to investigate the effect of different wavelengths of light on S. yoshimura hairy root. In this study, S. yoshimura hairy roots were inducted by Agrobacterium rhizogenes LBA1334. Different combinations of various wavelength light-emitting diodes (LEDs) were used to irritate S. yoshimura hairy root for 4 weeks. The content of harpagoside and cinnamic acid in the hairy root were analyzed by high performance liquid chromatography. We found that S. yoshimura hairy root irritated by red light and infrared light increase both fresh and dry weight, but reduce the amounts of harpagoside and cinnamic acid compared to dark-culture. On the other hand, S. yoshimurae hairy root irritated by blue light, green light and UV light reduce both fresh and dry weight, but increase the amounts of harpagoside and cinnamic acid. The results of this study is the first time showed that different wavelengths of light affect transformed hairy root growth and secondary metabolites production of S. yoshimura. These findings can be applied in the large production of harpagoside and cinnamic acid.
Yi-Chen Liu is currently a PhD student at National Chung-Hsing University, Taiwan.
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