Nikhilesh Dhuria1, Deep Bhattacharya2, Sunaina Bhaskar1 and Shruti Gaggar1
1Bombay College of Pharmacy, India 2University of Nebraska, USA
Posters-Accepted Abstracts: Nat Prod Chem Res
Studies have shown that Punica granatum (Lythraceae) and Psidium guajava (Myrtaceae) have remarkable biological and medicinal properties. This work explores and compares the antioxidant and anti-inflammatory activities of the methanolic extract of fruit peel of Punica granatum and ethanolic extract of the leaves of Psidium guajava. The extracts were administered orally (50 & 100 mg/ kg) for anti-inflammatory activity which was studied using rat paw edema test and the anti-lipid peroxidation test was carried using thio-barbituric acid reactive substances (TBARS) method. For lipid peroxidation inhibitory activity of P. guajava extract was found to reduce the TBARS formation better than their free form with an IC50 values of 16.86 and 13.09 μg/ml and for anti-inflammatory activity using rat paw edema method 1.2 (±0.002) at the third hour compared to control 2.5(±0.015) and standard diclofenac sodium 0.85 (±0.007) at 100 mg/kg dose. While for P. granatum antioxidant activity was found to have IC50 values 17.41 μg/ml for biomarker complex & 23.84 μg per ml for the plant extract complex. Anti-inflammatory activity was found maximum at third hour where the extract of High Yielding Polyphenolic Extract (HYPE) complex (21.95%) than their isolated form, it may be due to the presence of parent catechin, quercetin, myricetin and their metabolites. Both the extracts exhibited significant antioxidant and anti-inflammatory activity. The results demonstrated that P. granatum and P. guajava contains active constituents which possess antioxidant and antiinflammatory activity, justifying its popular uses.
Nikhilesh Dhuria is currently MPharm Student at Bombay College of Pharmacy.
Email: nikhilesh.dhuria@gmail.com
