Zohreh Bakhtiari1, Gholamreza Asghari2, Shekofeh Enteshari1 and Ahmad Reza Rahmati3
1Payam Noor University, Iran 2University of Medical Sciences, Iran 3Isfahan University of Medical Sciences, Iran
Posters-Accepted Abstracts: Nat Prod Chem Res
Introduction: Artemisinin is the most important anti-malaria medicine and its chemical synthesis is complex and costly. Due to the presence of gene manufacturer artemisinin in plant Artemisia aucheri Boiss and disable this gene and the abundance this plant in Iran, this experiment was designed with aim probability of producing artemisinin in cultured tissue in the presence of light stimuli. Experiment Method: For culture, was used of solid culture, Murashing & Skoog without growth regulators. Culture medium was placed in the culture room under sterile conditions, the temperature of 2±25 and different light conditions with different treatments. Lighting conditions was used of optical radiation 1000, 2000 and 3000 lux. In addition, it was assumed, radiation treatments UV and treatment of Darkness. For analyzing data and drawing diagrams, software SPSS version 20 and Excel programs was used. During the experiment, the resulting data, the average of three replicates ±SD and results based on one-way ANOVA test, Tukey test and a significant difference with p�0.05 were investigated. For detection of artemisinin, the dichloromethanolic extract was analyzed by TLC. Measure phenolic compounds and flavonoids were done into spectrophotometric method. Results: Seedlings in treatments under light 1000 and 3000 lux were produced artemisinin. Meanwhile, radiation-UV both the callus and the seedlings have affected and produced artemisinin. Effects of light stimuli on the chemical plant and its morphological characteristics, too, were confirmed. Conclusion: This study showed that increased exposure as well as the use of radiation UV, forcing Artemisia aucheri plant to produce artemisinin. In addition, the chemical plant and the shape of the light stimulus take effect.
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