Dongdong Wang and Shu Wang
Sichuan University, China
Posters-Accepted Abstracts: Nat Prod Chem Res
Bulbus Fritillariae cirrhosae (BFC) is widely used in China both for food and folk medicine due to its powerful biological activities. Firstly, this study was designed to examine the anti-proliferative activities of the different fractions from BFC in vitro by MTT assay. The results showed that Chloroform Extracts (CE) and the purified Total Alkaloids of BFC (TAF) exhibited stronger antiproliferative activity than the other fractions. We further determined the total alkaloids and three main alkaloids monomers content of CE and TAF by UV and HPLC-ELSD methods, respectively. Moreover, we assessed the antitumor activity of TAF in vivo and made preliminary investigation of its anti-tumor mechanism by histological and immuno-histochemical staining technique. These results demonstrate that TAF showed significant antitumor activity and low toxicity in vivo. Meanwhile, TAF significantly inhibited tumor angiogenesis and induced apoptosis by improvement of expression level of caspase-3. These results suggest that alkaloids of BFC could hold a good potential for use as an antitumor drug.
Email: wddong1988@hotmail.com
